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pe conjugated goat antimouse igg  (SouthernBiotech)


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    SouthernBiotech pe conjugated goat antimouse igg
    In vitro elimination of EGFR+ epithelial cancer cells (ECCs) by CD32A131R-chimeric receptor (CR) and CD16158F-CR T cells is associated with EGFR overexpression. (a) EGFR expression levels in the tumor cell lines as assessed by flow cytometry. The cells were incubated with 3 μg/ml of purified anti-EGFR antibody, washed and then stained with a phycoerythrin (PE)-conjugated <t>antimouse</t> <t>IgG</t> (gray filled histograms). The cells incubated with PE-conjugated antimouse IgG were used as a negative control (light gray filled histograms). Mean fluorescence intensity (MFI) is indicated for each cell line. (b) Spearman’s correlation between EGFR expression levels (MFI) in the HCT116, A549, MDA-MB-231 and MDA-MB-468 cell lines and the ECC elimination by CD16158F-CR T cells (left panel) and CD32A131R-CR T cells (right panel) in combination with cetuximab. A regression line is shown in black. Spearman’s rank correlation coefficient (r) was 0.6316 for CD16158F-CR and 0.533 for CD32A131R-CR. This is a cumulative analysis of five experiments separately performed: 116 = HCT116, 231 = MDA-MB-231, 468 = MDA-MB-468 and 549 = A549 cells.
    Pe Conjugated Goat Antimouse Igg, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe conjugated goat antimouse igg/product/SouthernBiotech
    Average 94 stars, based on 1 article reviews
    pe conjugated goat antimouse igg - by Bioz Stars, 2026-06
    94/100 stars

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    1) Product Images from "In vitro elimination of epidermal growth factor receptor-overexpressing cancer cells by CD32A-chimeric receptor T cells in combination with cetuximab or panitumumab"

    Article Title: In vitro elimination of epidermal growth factor receptor-overexpressing cancer cells by CD32A-chimeric receptor T cells in combination with cetuximab or panitumumab

    Journal: International journal of cancer

    doi: 10.1002/ijc.32663

    In vitro elimination of EGFR+ epithelial cancer cells (ECCs) by CD32A131R-chimeric receptor (CR) and CD16158F-CR T cells is associated with EGFR overexpression. (a) EGFR expression levels in the tumor cell lines as assessed by flow cytometry. The cells were incubated with 3 μg/ml of purified anti-EGFR antibody, washed and then stained with a phycoerythrin (PE)-conjugated antimouse IgG (gray filled histograms). The cells incubated with PE-conjugated antimouse IgG were used as a negative control (light gray filled histograms). Mean fluorescence intensity (MFI) is indicated for each cell line. (b) Spearman’s correlation between EGFR expression levels (MFI) in the HCT116, A549, MDA-MB-231 and MDA-MB-468 cell lines and the ECC elimination by CD16158F-CR T cells (left panel) and CD32A131R-CR T cells (right panel) in combination with cetuximab. A regression line is shown in black. Spearman’s rank correlation coefficient (r) was 0.6316 for CD16158F-CR and 0.533 for CD32A131R-CR. This is a cumulative analysis of five experiments separately performed: 116 = HCT116, 231 = MDA-MB-231, 468 = MDA-MB-468 and 549 = A549 cells.
    Figure Legend Snippet: In vitro elimination of EGFR+ epithelial cancer cells (ECCs) by CD32A131R-chimeric receptor (CR) and CD16158F-CR T cells is associated with EGFR overexpression. (a) EGFR expression levels in the tumor cell lines as assessed by flow cytometry. The cells were incubated with 3 μg/ml of purified anti-EGFR antibody, washed and then stained with a phycoerythrin (PE)-conjugated antimouse IgG (gray filled histograms). The cells incubated with PE-conjugated antimouse IgG were used as a negative control (light gray filled histograms). Mean fluorescence intensity (MFI) is indicated for each cell line. (b) Spearman’s correlation between EGFR expression levels (MFI) in the HCT116, A549, MDA-MB-231 and MDA-MB-468 cell lines and the ECC elimination by CD16158F-CR T cells (left panel) and CD32A131R-CR T cells (right panel) in combination with cetuximab. A regression line is shown in black. Spearman’s rank correlation coefficient (r) was 0.6316 for CD16158F-CR and 0.533 for CD32A131R-CR. This is a cumulative analysis of five experiments separately performed: 116 = HCT116, 231 = MDA-MB-231, 468 = MDA-MB-468 and 549 = A549 cells.

    Techniques Used: In Vitro, Over Expression, Expressing, Flow Cytometry, Incubation, Purification, Staining, Negative Control, Fluorescence



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    In vitro elimination of EGFR+ epithelial cancer cells (ECCs) by CD32A131R-chimeric receptor (CR) and CD16158F-CR T cells is associated with EGFR overexpression. (a) EGFR expression levels in the tumor cell lines as assessed by flow cytometry. The cells were incubated with 3 μg/ml of purified anti-EGFR antibody, washed and then stained with a phycoerythrin (PE)-conjugated <t>antimouse</t> <t>IgG</t> (gray filled histograms). The cells incubated with PE-conjugated antimouse IgG were used as a negative control (light gray filled histograms). Mean fluorescence intensity (MFI) is indicated for each cell line. (b) Spearman’s correlation between EGFR expression levels (MFI) in the HCT116, A549, MDA-MB-231 and MDA-MB-468 cell lines and the ECC elimination by CD16158F-CR T cells (left panel) and CD32A131R-CR T cells (right panel) in combination with cetuximab. A regression line is shown in black. Spearman’s rank correlation coefficient (r) was 0.6316 for CD16158F-CR and 0.533 for CD32A131R-CR. This is a cumulative analysis of five experiments separately performed: 116 = HCT116, 231 = MDA-MB-231, 468 = MDA-MB-468 and 549 = A549 cells.
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    Image Search Results


    In vitro elimination of EGFR+ epithelial cancer cells (ECCs) by CD32A131R-chimeric receptor (CR) and CD16158F-CR T cells is associated with EGFR overexpression. (a) EGFR expression levels in the tumor cell lines as assessed by flow cytometry. The cells were incubated with 3 μg/ml of purified anti-EGFR antibody, washed and then stained with a phycoerythrin (PE)-conjugated antimouse IgG (gray filled histograms). The cells incubated with PE-conjugated antimouse IgG were used as a negative control (light gray filled histograms). Mean fluorescence intensity (MFI) is indicated for each cell line. (b) Spearman’s correlation between EGFR expression levels (MFI) in the HCT116, A549, MDA-MB-231 and MDA-MB-468 cell lines and the ECC elimination by CD16158F-CR T cells (left panel) and CD32A131R-CR T cells (right panel) in combination with cetuximab. A regression line is shown in black. Spearman’s rank correlation coefficient (r) was 0.6316 for CD16158F-CR and 0.533 for CD32A131R-CR. This is a cumulative analysis of five experiments separately performed: 116 = HCT116, 231 = MDA-MB-231, 468 = MDA-MB-468 and 549 = A549 cells.

    Journal: International journal of cancer

    Article Title: In vitro elimination of epidermal growth factor receptor-overexpressing cancer cells by CD32A-chimeric receptor T cells in combination with cetuximab or panitumumab

    doi: 10.1002/ijc.32663

    Figure Lengend Snippet: In vitro elimination of EGFR+ epithelial cancer cells (ECCs) by CD32A131R-chimeric receptor (CR) and CD16158F-CR T cells is associated with EGFR overexpression. (a) EGFR expression levels in the tumor cell lines as assessed by flow cytometry. The cells were incubated with 3 μg/ml of purified anti-EGFR antibody, washed and then stained with a phycoerythrin (PE)-conjugated antimouse IgG (gray filled histograms). The cells incubated with PE-conjugated antimouse IgG were used as a negative control (light gray filled histograms). Mean fluorescence intensity (MFI) is indicated for each cell line. (b) Spearman’s correlation between EGFR expression levels (MFI) in the HCT116, A549, MDA-MB-231 and MDA-MB-468 cell lines and the ECC elimination by CD16158F-CR T cells (left panel) and CD32A131R-CR T cells (right panel) in combination with cetuximab. A regression line is shown in black. Spearman’s rank correlation coefficient (r) was 0.6316 for CD16158F-CR and 0.533 for CD32A131R-CR. This is a cumulative analysis of five experiments separately performed: 116 = HCT116, 231 = MDA-MB-231, 468 = MDA-MB-468 and 549 = A549 cells.

    Article Snippet: PE-conjugated goat antimouse IgG (1012-09) was purchased by Southern Biotech (Birmingham, AL).

    Techniques: In Vitro, Over Expression, Expressing, Flow Cytometry, Incubation, Purification, Staining, Negative Control, Fluorescence

    Combination of antibodies used to characterize natural killer cell phenotype.

    Journal: Frontiers in Immunology

    Article Title: Natural Killer Cells from Patients with Recombinase-Activating Gene and Non-Homologous End Joining Gene Defects Comprise a Higher Frequency of CD56 bright NKG2A +++ Cells, and Yet Display Increased Degranulation and Higher Perforin Content

    doi: 10.3389/fimmu.2017.00798

    Figure Lengend Snippet: Combination of antibodies used to characterize natural killer cell phenotype.

    Article Snippet: , PE/Cy7-conjugated goat anti-mouse IgG2b , Southern Biotech.

    Techniques: Control